Table 1 Primers sequences for qRT-PCR
From: Bivalent histone modifications during tooth development
Gene | Primer | Annealing temperature/°C | Fragment lengt/bp | Accession number |
|---|---|---|---|---|
SETD1A | F: 5′-AAAGGAGACATTGCCCACACA-3′ | 60 | 178 | NM_178029.3 |
R: 5′-GGAAACAGTTTTCCGGCGTT-3′ | ||||
SETD7 | F: 5′-TCCAAGAGTTCACACCTGCG-3′ | 60 | 167 | NM_080793.5 |
R: 5′-TTCCCCAAACTCGGCATTCA-3′ | ||||
KDM5A | F: 5′-GAGGGCGCAGAACTTTGAC-3′ | 59 | 161 | NM_145997.2 |
R: 5′-CTGCTAAATCAGTCCGCCAC-3′ | ||||
KDM5B | F: 5′-GGAAGAGACTGCGCTGTAAGT-3′ | 60 | 71 | NM_152895.2 |
R: 5′-GTGGGGGACTGAGTAGGGAG-3′ | ||||
EZH2 | F: 5′-TTCCATGCAACACCCAACACAT-3′ | 61 | 156 | NM_001146689.1 |
R: 5′-TGGGCGTTTAGGTGGTGTCT-3′ | ||||
JMJD3 | F: 5′-CAATCCCCGCAGAGCTTACC-3′ | 60 | 101 | NM_009483.1 |
R: 5′-TTCTACTGGAGGTGGTGCATT-3′ | ||||
UTX | F: 5′-TCTGCTGTAGCCCATAGGAC-3′ | 60 | 96 | NM_001017426.1 |
R: 5′-CAGCCAGATGGAGTTGGAAGT-3′ |
