Figure 8

Fluoride disturbs the expression of NFATc1 protein. The cell extracts were analyzed by immunoblotting with specific antibodies for NFATc1 and GAPDH. An equal amount of protein was loaded in each lane, as demonstrated by the level of GAPDH. Bound antibodies were visualized by chemiluminescence using the ECL Plus Western Blotting Detection System, and images were analyzed by a Luminescent Image Analyzer. The experiment was repeated three times. GAPDH, glyceraldehyde phosphate dehydrogenase; NFAT, nuclear factor of activated T cell; rRANKL, recombinant receptor activator of nuclear factor κB ligand.