Figure 2

Representative CLSM images of live/dead stained biofilms grown for two days on composites. The material name is indicated in each image, and the magnification of all images is the same as that in a. Live bacteria were stained green, and dead bacteria were stained red. Live and dead bacteria that co-localized appeared yellow or orange. Commercial composite and NACP nanocomposite without QAM had primarily live bacteria. Increasing the CL enhanced the antibacterial activity, with CL16 producingmostly red and orange staining. CL, chain length; NACP, nanoparticles of amorphous calcium phosphate; QAM, quaternary ammonium methacrylates.