Figure 5

Immunohistochemical staining. Original magnification ×400. (a-l) Collagen gel; (m-x) collagen gel with bFGF. Collagen gel stained with antibodies to vimentin, αSMA, Pan-CK, CD49f, c-kit and AQP5 at AOD 5 and AOD 7. In the control group, vimentin- and αSMA-positive cells were observed in the peripheral area of the collagen gel at AOD 5. Pan-CK-, CD49f-, c-kit- and AQP5-positive cells were not observed. In the bFGF group, CD49f- and c-kit-positive cells were observed at AOD 5, but AQP5-positive cells were not observed. In the both groups, αSMA-positive cells were observed in the inner area of the collagen gel at AOD 7. Vimentin-, Pan-CK-, CD49f-, c-kit- and AQP5-positive cells were observed in peripheral areas of the collagen gel. AOD after operation day; AQP5, Aquaporin 5; bFGF, basic fibroblast growth factor; αSMA, α-smooth muscle actin.