Figure 6

Example of immune-cytostained images of U2OS osteoblasts grown on Ti-P and ZrO2-MA surfaces. U2OS osteoblasts were grown for 24 h in DMEM/LG-complete at 37 °C in a humidified atmosphere with 5% CO2 and subsequently grown for 48 h in anaerobic conditions in modified culture medium in absence and presence of a challenge with P. intermedia. Bar markers indicate 100 μm. DMEM/LG, Dulbecco's modified Eagle's medium low glucose.