Figure 7
From: Phenotypic research on senile osteoporosis caused by SIRT6 deficiency
Day 7 of cell culture for osteoclastogenesis. (a) Trap staining for osteoclast. (b) The cell number of osteoclast per well. The 48-well plates were used for cell culture. Large cells with multi-nuclei and foamy cytoplasm were quantified as mature osteoclasts. Other Trap+ but smaller cells with more than two nuclei were marked as individual osteoclasts, such as the cells indicated by black arrows in figure. The data are representative of six independent experiments. *P<0.05. KO, knock out; OC, osteoclast; Trap, tartrate-resistant acid phosphatase; WT, wild type.
