Figure 4 | International Journal of Oral Science

Figure 4

From: Intragingival injection of Porphyromonas gingivalis-derived lipopolysaccharide induces a transient increase in gingival tumour necrosis factor-α, but not interleukin-6, in anaesthetised rats

Figure 4

RT‐PCR analysis of expression of TLR2 and TLR4 mRNA in rat gingival tissue. Total RNA was extracted from rat gingival tissue. After cDNA was generated, PCR was performed with specific primers against TLR2, TLR4 and GAPDH. The PCR products were loaded onto a 1.5% agarose gel and photographed. The expected PCR products of TLR‐2, TLR‐4 and GAPDH are 177, 105 and 155 bp, respectively. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PCR, polymerase chain reaction; RT‐PCR, reverse transcriptase‐polymerase chain reaction; TLR, Toll‐like receptor.

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