Figure 2
From: Engineered three-dimensional rabbit oral epithelial–mesenchymal–muscular hybrid sheets
Multi-differentiation of rOMMCs and rOMYCs. (a) Alizarin red S staining of non-induced (upper panel) or osteogenic-induced (lower panel) rOMMCs. (b) Oil red O staining of non-induced (upper panel) or adipogenic-induced (lower panel) rOMMCs. (c) Safranin O staining of non-induced (upper panel) or chondrogenic-induced (lower panel) rOMMCs. Scale bars, 50 μm. (d) Phase-contrast image of high-density culture of rOMYCs. (e) Expression of Pax7, CD34, desmin, and MyoD by RT-PCR in high-density culture of rOMYCs. GAPDH was used as an internal control. (f) Immunocytochemistry of desmin (red) in high-density non-induced (upper panel) or myogenic-induced rOMYCs (lower panel). Nuclei were stained with 4,6-diamidino-2-phenylindole. (g) Alizarin red S staining in non-induced (upper panel) and osteogenic-induced (lower panel) rOMYCs. (h) Oil red O staining in non-induced (upper panel) and adipogenic-induced (lower panel) rOMYCs. Scale bars, 50 μm. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; rOMMCs, rabbit oral mucosa mesenchymal tissues; rOMYCs, rabbit oral myoblasts.
