Figure 3
From: Engineered three-dimensional rabbit oral epithelial–mesenchymal–muscular hybrid sheets
Alteration of expressed epithelial and muscular structural proteins in hybrid sheets. (a) Double staining of rabbit hybrid sheets after 1, 3, 5, and 7 days with K4 (green) and desmin (red). Nuclei were stained with DAPI. Scale bars, 50 μm. (b) Double staining of rabbit hybrid sheets after 1, 3, 5, and 7 days with K13 (green) and desmin (red). Nuclei were stained with DAPI. Scale bars, 50 μm. (c) K13 western blot of rabbit hybrid sheets after 1, 3, 5, and 7 days. β-Actin was used as an internal control. (d) Relative expression of K13 (K13/β-actin) protein (n=4). (e) Desmin western blot of rabbit hybrid sheets after 1, 3, 5, and 7 days. β-Actin was used as an internal control. (f) Relative expression of desmin (desmin/β-actin) protein (P<0.05, n=4). DAPI, 4,6-diamidino-2-phenylindole.
