Figure 3

Total ROS production and extracellular ROS production in unstimulated samples and samples stimulated with F. nucleatum , assessed using luminol and isoluminol, respectively. (a) Total ROS; (b) extracellular ROS. Maximal RLUs were recorded and corrected for the number of viable cells. Data are means RLUs±standard deviation, *comparison oPMNs and cPMNs, P<0.001; **comparison unstimulated and F. nucleatum, P<0.001. cPMN, circulatory polymorphonuclear neutrophils; oPMN, oral polymorphonuclear neutrophils; RLUs, relative light units; ROS, reactive oxygen species.