Table 1 The sequences of the gene-specific primers used for the real-time qPCR analysis
From: Globoside accelerates the differentiation of dental epithelial cells into ameloblasts
Gene | Forward primer | Reverse primer | Size/bp |
|---|---|---|---|
Mouse/rat Amel | 5′-GATGGCTGCACCACCAAATC-3′ | 5′-CTGAAGGGTGTGACTCGGG-3 | 65 |
Mouse Enam | 5′-TGCAGAAATCCGACTTCTCCT-3′ | 5′-CATCTGGAATGGCATGGCA-3′ | 114 |
Rat Enam | 5′-ACCGAAAGTCCTGACACATTGG-3′ | 5′-CCTGAAGCAGTAAACAGCCGTG -3′ | 154 |
Mouse/rat Mmp-20 | 5′-GGCGAGATGGTGGCAAGAG-3′ | 5′-CTGGGAAGAGGCGGTAGTT-3′ | 166 |
Mouse Ambn | 5′-ATGAAGGGCCTGATCCTGTTC-3′ | 5′-GTCTCATTGTCTCAAGGCTCAAA-3′ | 130 |
Mouse/rat Epfn | 5′-CCTTTCTATTTCACCCTCCCCTG-3′ | 5′-CCACCACCTCATCTCTGCTTTCTC-3′ | 282/248 |
Rat Runx2 | 5′-CGGGAATGATGAGAACTACTC-3′ | 5′-TGAAACTCTTGCCTCGTCC-3′ | 118 |
Mouse/rat GAPDH | 5′-CCATCACCATCTTCCAGGAG -3′ | 5′-GCATGGACTGTGGTCATGAG-3′ | 322 |
