Figure 1

Representative specimen of the RI/CR combination group. (a) Macroscopic view of incipient occlusal lesion before treatment (note location of the highest DIAGNOdent pen value measured). (b) Radiation-free DIAGNOcam image of the same specimen, depicting the extent of the carious lesion, along with the sectional plane (indicated by the blue dotted line). (c) Macroscopic view of the same specimen, cut through the central grove, and depicting the fissure caries. (d) Corresponding CLSM micrograph (× 4), revealing the deep and homogeneous penetration of the resin infiltrant into the lesion body, visualized by the fluorescently labelled (RITC incorporated by polymerization) resin infiltrant, at the same time depicting the adhesive seal on the fissure walls. CLSM, confocal laser-scanning microscopy; CR, composite resin; RI, resin infiltrant; RITC, rhodamine B isothiocyanate.