Figure 4

CLSM micrographs (× 4) depicting a specimen from the CR group. (a) At the bottom of the fissure, an unfilled area (void) is clearly visible (see blue arrows), along with the fluorescently labelled (FITC) porous volume of the carious lesion. (b) Microleakage visualized by fluorescent labelling (RITC, see yellow arrows). CLSM, confocal laser-scanning microscopy; CR, composite resin; FITC, fluorescein isothiocyanate; RITC, rhodamine B isothiocyanate.