Figure 5 | International Journal of Oral Science

Figure 5

From: An early report: a modified porphyrin-linked metronidazole targeting intracellular Porphyromonas gingivalis in cultured oral epithelial cells

Figure 5

Live cell imaging of 3D reconstruction and colocalization of porphyrin adducts and pathogen. (a) Live cell imaging of z-stack projection at 3 h culture showed porphyrin-linked metronidazole adducts (in red, nuclei in blue) within the cytoplasm of epithelial H413-1 cells by confocal laser scanning microscopy. (b) 3D reconstruction with sliced images of one cell confirmed the compound (in red, nuclei in blue) localized in the cytoplasm. (c) Adherent and intracellular P. gingivalis shown at 1.5 h bacterial invasion to the cells (bacteria in green, nuclei in blue). (d) Intracellular P. gingivalis showing perinuclear spots in green (grey colour was a differential interference contrast (DIC)) after the killing of extracellular bacteria by combined antibiotics for additional 1 h. (e) Demonstration of the porphyrin adducts (in red) colocalized with P. gingivalis (in green, d) within the cytoplasm around nuclei (perinuclear) of epithelial cells in yellow/orange colour (f) at additional 1.5 h culture of this compound.

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