Figure 4
From: The importance of a potential phosphorylation site in enamelin on enamel formation

The distribution and intermediate fragment patterns of AMBN in 6-d-old ENAMRgsc514 mice. (a)–(l) Immunohistochemical staining of AMBN in the first lower molars of 6-d-old mice showed that the distribution patterns of the C-terminal and N-terminal fragments of AMBN had no apparent differences among the WT and ENAMRgsc514 mice. (m) Western immunoblotting revealed that a ~15 kDa C-terminal fragment of AMBN (red arrowhead) was absent in the tooth of the ENAMRgsc514 homozygotes. Scale bars: 200 μm in a, c, e, g, i, and k. 50 μm in b, d, f, h, j, and l.