Figure 5
From: The importance of a potential phosphorylation site in enamelin on enamel formation
The distribution and intermediate fragment patterns of AMEL in 6-d-old ENAMRgsc514 mice. (a)–(f) Immunohistochemical staining of AMEL in the first lower molars of 6-d-old mice showed that the distribution patterns of AMEL had no apparent differences among the WT and ENAMRgsc514 mice. (g) Western immunoblotting showed similar fragment patterns of AMEL among the WT and ENAMRgsc514 mice, although the ENAMRgsc514 homozygotes seemingly had less aggregated AMEL at ~50 kDa. In addition, the ENAMRgsc514 mice showed fewer amounts of ~20 kDa AMEL fragments compared to the WT mice. Scale bars: 200 μm in a, c, and e. 50 μm in b, d, and f.
