Figure 3 | International Journal of Oral Science

Figure 3

From: Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis, Porphyromonas gingivalis lipopolysaccharide, and extracellular adenosine triphosphate

Figure 3

Tight junctions' staining. Occludin, JAM-A, claudin-1, claudin-4, claudin-15, and ZO-1 staining (a–x): H413 clone-1 cells treated with P. gingivalis infection (a–f), P. gingivalis LPS stimulation (g–l), and ATP plus P. gingivalis (m–r), or P. gingivalis LPS (s–x) for 2 h. Only moderate expression was observed for claudin-4 (d) after P. gingivalis infection; weak staining was detected for claudin-1 (i), and the majority of immune-reactivity was detected for claudin-15 (k) and ZO-1 (l) with P. gingivalis LPS treatment. After ATP pretreatment for 3 h, significant staining for occludin, JAM-A, claudin-1, claudin-4, claudin-15, and ZO-1 were observed in both groups (m–x), control (y), ATP-control (z), error bar, 20 μm. There were similar patterns with different treatments for 4 h (data not shown). ATP, adenosine triphosphate; JAM, junctional adhesion molecule; LPS, lipopolysaccharide; ZO, zonula occludens.

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