Table 1 Summary of nanoSIMS analyses, cell dimensions and estimates of N2 fixation rates and growth rates
From: Nitrogen fixation and transfer in open ocean diatom–cyanobacterial symbioses
Diatom symbiont | Time (h) | ROIs (n) | AT % (15N/14N) | Biovolume (μm3) | N2 fix rate (fmol N per cell h−1)a | Growth rate (division per day) |
|---|---|---|---|---|---|---|
Hemiaulus | 3 | 0.3789 | 4.22 × 103 | 0.22 | ||
Heterocyst 1 | 1 | 0.3726 | 164 | 21.0 | ||
Vegetative | 0.5 | 1 | 0.3790 | 8.72 | 0.22 | |
Heterocyst 2 | 1 | 0.3812 | 37.4 | |||
Vegetative | 1 | 0.3806 | 16.4 | 0.23 | ||
Hemiaulus | 3 | 0.3651 | 3.49 × 103 | 0.06 | ||
Heterocyst | 1 | 1 | 0.3761 | 102 | 5.88 | |
Vegetative | 2 | 0.3607 | 8.32 | 0.05 | ||
Hemiaulus | 4 | 0.3759 | 1.87 × 103 | 0.10 | ||
Heterocyst | 1 | 1 | 0.3764 | 85.1 | 8.94 | |
Vegetative | 2 | 0.3737 | 15.5 | 0.09 | ||
Hemiaulus | 0.3595 | 1.91 × 103 | 0.03 | |||
Heterocyst | 1 | 1 | 0.3623 | 34.3 | 2.90 | |
Vegetative | 2 | 0.3657 | 8.62 | 0.06 | ||
Hemiaulus | 6 | 0.4378 | 4.63 × 103 | 0.11 | ||
Heterocyst | 1 | 0.4238 | 50.0 | 9.45 | ||
Vegetative | 3 | 3 | 0.4362 | 6.0 | ||
Heterocyst | 1 | 0.4508 | 45.6 | 0.10 | ||
Hemiaulus | 8 | 0.4576 | 4.23 × 103 | 0.13 | ||
Heterocyst 1 | 3 | 1 | 0.4104 | 72.3 | 10.6 | |
Vegetative | 2 | 0.4792 | 29.6 | 0.16 | ||
Heterocyst 2 | 1 | 0.4706 | 44.9 | |||
Hemiaulus | 6 | 0.4523 | 4.32 × 103 | 0.12 | ||
Heterocyst 1 | 1 | 0.4118 | 41.1 | |||
Vegetative | 3 | 1 | 0.4951 | 11.7 | 10.3 | 0.10 |
Heterocyst 2 | 1 | 0.4596 | 55.0 | |||
Vegetative | 1 | 0.4485 | 13.4 | 0.11 | ||
Hemiaulus | 12 | 5 | 0.4739 | 1.56 × 103 | 0.04 | |
Heterocyst | 1 | 0.4572 | 13.1 | 1.41 | ||
Hemiaulus | 5 | 0.4519 | 1.49 × 103 | 0.04 | ||
Heterocyst | 12 | 1 | 0.4826 | 10.4 | 1.15 | |
Vegetative | 2 | 0.4748 | 5.77 | 0.04 | ||
FL Calothrix | ||||||
Heterocyst | 1 | 1 | 0.3570 | 35.8 | 0.17 | 0.06 |
Vgetative | 6 | 0.3679 | 28.5 | |||
FL Richelia | ||||||
Heterocyst | 12 | 1 | 0.4575 | 44.5 | 0.12 | |
Vegetative | 6 | 0.7415 | 45.1 | 0.11 | ||
FL Crocosphaera | 12 | 19 | 0.3561–0.4519 | 65–2.66 × 102 | 0.02–2.39 | 0.001–0.15 |
Hemiaulus | 3 | 0.4032 | 2.39 × 104 | |||
Heterocyst 1 | 0 | 1 | 0.3632 | 9.10 × 102 | ||
Heterocyst 2 | 1 | 0.3951 | 3.17 × 102 | |||
Hemiaulus | 3 | 0.3494 | 2.84 × 104 | |||
Heterocyst 1 | 1 | 0.3689 | 6.84 × 102 | |||
Heterocyst 2 | 0 | 1 | 0.3743 | 4.37 × 102 | ||
vegetative | 1 | 0.3617 | 2.34 × 102 | |||
Hemiaulus | 4 | 4.4440 | 4.41 × 103 | 0.43 | ||
Heterocyst | 24 | 1 | 1.7900 | 75.3 | 47.8 | |
Vegetative | 2 | 4.4600 | 18.9 | 0.43 | ||
Hemiaulus | 3 | 4.6600 | 3.12 × 103 | 0.45 | ||
Heterocyst | 24 | 1 | 2.6810 | 71.0 | 38.7 | |
Vegetative | 3 | 4.7430 | 27.4 | 0.46 | ||
Hemiaulus | 6 | 4.5960 | 4.31 × 103 | 0.45 | ||
Heterocyst 1 | 1 | 4.7068 | 38.7 | 48.7 | ||
Vegetative | 24 | 1 | 4.7815 | 7.37 | 0.47 | |
Heterocyst 2 | 1 | 4.9327 | 48.6 | |||
Vegetative | 2 | 4.9693 | 28.8 | 0.49 | ||
Hemiaulus | 4 | 3.1628 | 2.12 × 103 | 0.15 | ||
heterocyst 1 | 1 | 3.1141 | 39.1 | 9.43 | ||
vegetative | 48 | 2 | 3.8720 | 41.6 | 0.18 | |
heterocyst 2 | 1 | 3.9909 | 35.8 | |||
Hemiaulus | 3 | 3.0170 | 4.30 × 103 | 0.14 | ||
Heterocyst | 48 | 1 | 3.4009 | 41.9 | 15.3 | |
Vegetative | 4 | 3.5284 | 23.4 | 0.17 | ||
Hemiaulus | 3 | 3.0506 | 4.68 × 103 | 0.14 | ||
Heterocyst | 48 | 1 | 3.6647 | 12.1 × 101 | 16.5 | |
Vegetative | 3 | 3.5281 | 16.6 | 0.33 | ||
Hemiaulus | 4 | 9.7529 | 4.40 × 103 | 0.48 | ||
Heterocyst 1 | 1 | 9.8114 | 74.0 | 50.4 | ||
Vegetative | 76 | 1 | 10.655 | 14.0 | 0.48 | |
Heterocyst 2 | 1 | 11.652 | 68.9 | |||
Vegetative | 1 | 11.891 | 33.2 | 0.59 | ||
Hemiaulus | 3 | 9.6706 | 4.33 × 103 | 0.48 | ||
Heterocyst | 76 | 1 | 3.1086 | 94.8 | 49.2 | |
Vegetative | 1 | 9.0384 | 41.6 | 0.44 | ||
Chaetoceros | 12 | 0.3593 | nm | |||
Heterocyst 1 | 1 | 0.3056 | 11.9 | |||
Vegetative | 0 | 13 | 0.3339 | 29.6 | ||
Heterocyst 2 | 1 | 0.3417 | 15.6 | |||
Vegetative | 5 | 0.3398 | 44.0 | |||
Chaetoceros | 3 | 7.6500 | 2.17 × 104 | 0.38 | ||
Heterocyst 1 | 1 | 4.3552 | 91.3 | 71.5 | ||
Vegetative | 76 | 3 | 7.0634 | 67.0 | 0.35 | |
Heterocyst 2 | 1 | 6.5394 | 70.9 | |||
Vegetative | 10 | 6.9387 | 66.7 | 0.32 | ||
Climacodium | 0 | 2 | 0.3797 | nm | ||
Crocosphaera | 4 | 0.3710 | 55.9 | |||
Climacodium | 76 | 11 | 3.1657 | 1.56 × 105 | 6.03 | 0.37 |
Crocosphaera | 28 | 5.3341 | 65.4 | 1.62 | 0.26 | |
