Figure 3

Phylogenetic affiliation of N. hollandicus. 16S rRNA-based Bayesian inference tree (s.d.=0.009202) showing the affiliation of N. hollandicus (boldface) with the class Thermomicrobia (gray box) of the phylum Chloroflexi. Pie charts indicate statistical support of nodes based on bootstrap analysis or Bayesian inference, respectively. Conflicting topologies among the different methods are also indicated. Representatives of the phylum Actinobacteria were used as outgroup. The scale bar represents 10% estimated sequence divergence. MB, Bayesian inference; ML, maximum likelihood; MP, maximum parsimony; NJ, neighbor joining.