Figure 4

Synteny within acI genomes. The following scaffolds were compared using gapped TBLASTX to identify putative homologs: (top) acI-A6 fosmid K004 from Lake Kinneret (GQ387492); (middle) acI-B1 scaffold0006 from Lake Mendota SCGC AAA027-L06; (bottom) acI-B1 scaffold JCVI_SCAF_1097207254344 from Lake Gatun GOS20. The scaffolds from Lake Kinneret and Lake Gatun were re-annotated in the IMG pipeline to ensure consistency in gene calling and nomenclature. The blast hit color-coding represents percent protein sequence identity, except for the rRNA genes, which were analyzed separately using BLASTN. Open reading frames encoding tRNA synthetases are highlighted in orange. The alanine dehydrogenase that appears to be missing in the SAG was found elsewhere on another scaffold (see text), but none of the other regions unique to the Lake Kinneret fosmid were found in the SAG.