Table 2 Metagenomic and metatranscriptomic reads assigned to enzyme classes that catalyse key steps in anaerobic respiration and fermentation, and fermentative pathways

From: Organic carbon transformations in high-Arctic peat soils: key functions and microorganisms

Pathway/function

E.C.

Gene

Metagenomes

Metatranscriptomes

   

S2a

S2b

K1a

K1b

K1c

S1a+S2a

K1a+K2a

Nitrate reduction

1.9.6.1

napA

1

6

7

4

1

0

0

Nitrate reduction

1.7.99.4

narG

10

6

14

19

28

0

0

Nitrite reduction

1.7.2.1

nirK

1

1

2

3

3

0

0

Nitrite reduction

1.7.2.1

nirS

0

1

0

2

1

0

2

Nitric oxide reduction

1.7.99.7

norB

1

4

3

2

4

0

1

Nitrous oxide reduction

1.7.99.6

nosZ

3

3

1

2

1

0

0

Sulphate reduction

1.8.99.3

dsrA

0

1

1

4

11

0

0

Fermentation: [FeFe] hydrogenase

1.12.7.2

hydA

2

9

0

7

8

0

0

Homoacetogenesis

6.3.4.3

fthfs

6

12

7

10

23

0

0

Methanogenesis

2.8.4.1

mcrA

0

0

1

0

2

0

0

Acetoin, butanediol metabolism

  

55

92

60

85

133

8

8

Acetone, butanol, ethanol synthesis

  

243

298

319

280

346

17

17

Acetyl-CoA fermentation to butyrate

  

310

362

406

329

407

16

22

Butanol biosynthesis

  

235

293

315

263

308

9

19

Fermentations: lactate

  

52

76

56

74

104

2

3

Fermentations: mixed acid

  

107

157

121

131

190

5

8

Tot. ass. sequences

  

69 176

63 527

85 664

54 889

72 166

6315

6205

  1. First column shows function or main pathway, second column shows the enzyme class entry of the ExPASY bioinformatics resource, while third column shows the gene abbreviation. Merged biological replicates for the two sites Solvatn (S1a+S2a) and Knudsenheia (K1a+K2a) are listed under metatranscriptomes. Total ass. sequences, the number of sequences assigned a function by MEGAN analysis. Sequences assigned to pathways were annotated using (MG)-RAST (see Materials and methods for details).