Table 1 Origin of the 10 new vampyrellid strains isolated in this study

From: Vampires in the oceans: predatory cercozoan amoebae in marine habitats

Isolate

Collection date

Geographic origin

Habitat

Sample type

Phylogenetic position a

NVam1 (Penardia sp.)

Spring 2010

North Carolina, USA

Brackish

Muddy sediment, Cape Fear River, near the estuary

Clade C, subclade P

CAraX

Spring 2009

California, USA

Marine

Sandy sediment, San Francisco Ocean Beach

Clade B, lineage B5

MVa1x (Thalassomyxa sp.)

Summer 2011

Majorca, Spain

Marine

Diatom-rich coastal sandy sediment

Clade B, subclade T

KibAr

Autumn 2007

Cumbria, UKb

Marine

Coastal sediment and rock scrapings, Walney Island

Clade B, subclade T

En42C

Autumn 2007

Cumbria, UKb

Marine

Coastal sediment and rock scrapings, Walney Island

Clade B, subclade T

V1ld4 c

Autumn 2010

Cumbria, UKb

Marine

Coastal sediment and rock scrapings, Walney Island

Clade B, subclade T

V2ld4

Autumn 2010

Cumbria, UKb

Marine

Coastal sediment and rock scrapings, Walney Island

Clade B, subclade T

V1ld9 d

Autumn 2010

Cumbria, UKb

Marine

Coastal sediment and rock scrapings, Walney Island

Clade B, subclade T

WaAra

Spring 2008

Wales, UK

Freshwater

Mixture of moss and lichens from a garden in Gregynog

Clade A, Leptophryidae

BAra1

Summer 2009

Amazon basin, Brazil

Freshwater

Mixture of aquatic plants from a small Rio Negro tributary

Clade A, Leptophryidae

  1. aSee Figures 4 and 5.
  2. bSamples collected in the same location.
  3. cIsolates V1ld4 and KibAr have identical small-subunit ribosomal DNA gene sequences.
  4. dIsolates V1ld9 and En42C have <0.3% overall small-subunit ribosomal DNA gene sequence divergence.