Figure 1

The phylogenetic tree of uncultivated MCG discussed in the text. The tree was constructed from the alignment of >900 unambiguously aligned base pairs using MAFFT followed by Maximum likelihood method by RAxML with the GTRGAMMA model. The stability of the topology was evaluated by bootstrapping (100 replicates). The resulting bootstrap values are indicated at each node in the tree. The names of MCG groups (MCG-A to -G, and class 1–17) were modified based on Jiang et al.’s classification (Jiang et al., 2011) and Kubo et al.’s classification (Kubo et al., 2012), respectively.