Figure 5
Clostridial flagellin transcript and gene copies during colitis and recovery. RNA and DNA were recovered from the same nucleic acids preparation, RNA was reverse transcribed into single-stranded cDNA. Gene and transcript copies were determined with qPCR. The ratio of flagellin to gene copies was calculated for individual mice and log-transformed. Means were compared by One-Way ANOVA, different letters indicate significance (P<0.05).
