Table 1 Growth conditions for B. cenocepacia transcriptomic reference set

From: The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia

Experiment a

Test condition

Control condition (control number) b

Stationary phase in minimal medium

BSM

Log phase in BSM (4)

Stationary phase in nutrient-rich medium

LB

Log phase in LB (1)

Low oxygen concentration

LB, atmosphere with 6% oxygen

Fully aerated LB (2)

Oxidative stress (inorganic peroxide)

LB, 0.15% H2O2, 15 min

LB without added peroxide (3)

Oxidative stress (organic peroxide)

LB, 0.001% tertiary butyl hydroperoxide, 15 min

LB without added peroxide (3)

Heat stress

LB, 42.5 °C, 1 h

LB at 37 °C (3)

Physiological temperature

LB, 20 °C

LB at 37 °C (2)

Low iron concentration

BSM, Fe content 0.064 p.p.m. (1.2 μM)

BSM, Fe content 2.45 p.p.m.=44 μM (4)

Low pH

BSM, pH 5.5

BSM, pH 7.0 (4)

  1. Abbreviations: BSM, basal salts medium; LB, Luria Bertani broth
  2. aCultures were incubated fully aerated shaking at 150 r.p.m. and at 37 °C if not stated otherwise.
  3. bControls we run for each condition as indicated by the control number; this number also corresponds to the control conditions shown in Figure 1.
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