Figure 3 | The ISME Journal

Figure 3

From: Long-term nitrogen fertilization of paddy soil shifts iron-reducing microbial community revealed by RNA-13C-acetate probing coupled with pyrosequencing

Figure 3

Quantitative distribution of density-resolved bacterial 16S rRNAs obtained from non-fertilized (NF; a–c) and N-fertilized (N; d–f) soil slurries treated with ferrihydrite (FER; a and d), goethite (GOE; b and e) and control (CTR; c and f) after 4-day anoxic incubation with either labeled (13C) or unlabeled (12C) acetate as the substrate. Bacterial template distribution within rRNA gradient fractions was quantified with real-time reverse transcription PCR. The normalized data are the ratio of the copy number in each gradient fraction to the maximum quantities from each treatment. The rRNA fractions subjected to pyrosequencing analysis are marked with arrows.

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