Table 2 The fate of 13C-acetate after 4-day anoxic incubations of NF and N soil slurries in the treatments with FER, GOE and CTR

From: Long-term nitrogen fertilization of paddy soil shifts iron-reducing microbial community revealed by RNA-13C-acetate probing coupled with pyrosequencing

 

FER

GOE

CTR

 

NF

N

NF

N

NF

N

13C from acetate added (μmol)

60

60

60

60

60

60

Gaseous 13CH4 (μmol)

9.9

7.6

13

9.5

14

11

Gaseous 13CO2 (μmol)

2.6

4.1

2.7

4.7

3.1

5.0

Dissolved 13CO2a (μmol)

3.3 (7.7)b

8.0 (7.9)

2.8 (7.6)

7.3 (7.8)

2.6 (7.5)

5.1 (7.6)

13C recoveryc (%)

27

33

31

36

33

35

  1. Abbreviations: CTR, control; FER, ferrihydrite; GOE, goethite; N, N-fertilized; NF, non-fertilized.
  2. aDissolved 13CO2 components including carbonic acid, bicarbonate and carbonate were estimated by using the gaseous 13CO2 concentration and the pH of the soil slurries after the incubation.
  3. bThe pH of soil slurries in each treatment after the incubation is presented in the parenthesis.
  4. c13C recovery is the total proportion of 13C from gaseous and dissolved products to the 13C from acetate added.