Table 2 Information of the 11 draft genomes assembled from metagenomic and metatranscriptomic data of the AMD community

From: Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics

Draft genome

Closely related organism

Total bases

Number of

GC content

Completeness

16 S rDNA

  

(bp)

contigs

(%)

(%)d

sequence

FKB1

Acidithiobacillus ferrooxidans a

2 453 291

364

58.0

80.9

No

FKB2

Acidithiobacillus thiooxidans a

2 643 526

330

53.2

85.5

No

FKB3

Leptospirillum rubarum (group II)a

2 067 509

295

57.7

77.6

No

FKB4

Leptospirillum ferrodiazotrophum (group III)a

2 593 804

235

59.0

91.8

No

FKB5

Acidiphilium cryptum a , c

3 468 182

361

66.7

91.3

Yes

FKB6

Alicyclobacillus acidocaldarius a , b

2 449 390

286

45.5

75.7

No

FKB7

Ferrovum spp.c

2 983 188

276

40.1

79.0

Yes

FKA8

Candidatus Micrarchaeum acidiphilum ARMAN-2a,c

1 266 728

179

43.5

84.9

Yes

FKA9

Candidatus Parvarchaeum acidiphilum ARMAN-4a,c

1 223 344

203

35.9

79.1

Yes

FKA10

Candidatus Parvarchaeum acidophilus ARMAN-5a

1 041 772

193

39.9

74.1

No

FKA11

Picrophilus torridus a

1 113 980

213

40.9

53.2

No

  1. aThe taxonomic information was achieved by comparing contigs against NCBI-nr database using BLASTx.
  2. bThe nearest neighbor in maximum-likelihood phylogenies constructed with RAxML (v7.2.7) by combining 31 universal marker genes was identified as the closest organism.
  3. cThe taxonomic information was obtained by comparing 16 S rDNA sequence against NCBI-nt database using BLASTn.
  4. dThe completeness of each genome was estimated by the ratio of core genes observed in each genome and the corresponding pan-genome (see Supplementary Methods for details).