Figure 3
From: Life in an unusual intracellular niche: a bacterial symbiont infecting the nucleus of amoebae
Infection cycle of ‘Candidatus Nucleicultrix amoebiphila’ in Hartmannella sp. FS5. Infection was monitored over a course of 144 h and visualized with FISH using probes CBR125 (orange) and EUK516-Cy5 (gray). Single bacterium starts colonizing the nuclear compartment within the first 6 h post infection; the nucleus is completely filled with bacteria after 96 h. Fully infected and highly enlarged nuclei can be observed after 120 h. The course of infection of Acanthamoeba sp. cells is similar (Supplementary Figure S7). Bar, 5 μm.
