Figure 4
From: Life in an unusual intracellular niche: a bacterial symbiont infecting the nucleus of amoebae
Modes of transmission of ‘Candidatus Nucleicultrix amoebiphila’. Key events during transmission of Nucleicultrix in Hartmannella sp. visualized by FISH are shown in the left panel. Bacteria and amoebae were labeled with probes CBR125 (orange) and EUK516 (gray), respectively. A dividing Hartmannella trophozoite is shown (i) in which the distribution of Nucleicultrix among both daughter cells can be seen. Note the single bacterial cell (arrows) within the amoeba nucleus undergoing segregation. Host cell lysis (ii) occurs only rarely in Hartmannella sp.; bar, 10 μm. A model of the life cycle and transmission of Nucleicultrix in amoebae is shown in the right panel. Although vertical transmission is predominant in Hartmannella sp., host lysis and horizontal transmission is the major route of Nucleicultrix in acanthamoebae.
