Figure 5
From: Microbial, host and xenobiotic diversity in the cystic fibrosis sputum metabolome
(a) The molecular network cluster of sphingolipids and related molecules in sputum data. Node size was scaled according to their abundances using the Cytoscape software. Nodes identified in the random forests VIP are shown as squares, and round nodes were not identified by random forests. Annotation of sphingomyelin (d18:1/16:0) was verified with a purchased standard and its chemical structure is shown. Putative annotations of other nodes in the cluster are according to the LipidMaps database and guided by MS/MS fragmentation spectra. Abundances of the metabolites in the cluster are shown using boxplots from CF and non-CF area under the curve data with attention to the most abundant molecules. (b) Area under the curve abundances of ceramide (Cer), sphingomyelin (SM) and C18 phosphocholine (PC) in CF and non-CF sputa. Statistically significant differences were determined using a Wilcoxon rank-sum test.
