Table 3 Sequence-based comparisons of strains MWH-MoK4 and QLW-P1DMWA-1

From: Complete ecological isolation and cryptic diversity in Polynucleobacter bacteria not resolved by 16S rRNA gene sequences

Compared feature

Value

16S rRNA similarity, entire genea (%)

99.7

16S rRNA similarity, V3–V5 regionb (%)

99.8

16S-23S ITS similarity (%)

96.1

glnA similarityc (%)

88.7

Shared genes (% of total)

71.0

AAI (shared genes/proteins) (%)

84.2

Two-way AAI (1557 proteins)d (%)

80.7

ANIb (JSpecies) (%)

75.6

Two-way ANI (Kostas lab) (%)

79.8

DNA–DNA hybridization estimatese (GGDC 2.0) (%)

19.3–29.3

  1. Abbreviations: AAI, average amino-acid identity; ANI, average nucleotide identity; rRNA, ribosomal RNA.
  2. a1527 bp.
  3. bPartial gene encompassing the V3 to V5 region; typically sequenced in high-throughput studies; 584 bp.
  4. cPartial glnA sequences, 603 bp.
  5. dAverage amino-acid identity calculator, Kostas lab.
  6. eThree estimates by three different algorithms.