Table 1 List of organisms screened for fatty acid synthesis enzymes in marine microbial eukaryotes

Positive libraries

 Alveolates

Dinoflagellates: Alexandrium andersonii (CCMP2222-1); Alexandrium fundayense (38/3-1); Alexandrium margalefi (CS322-1); Alexandrium monilatum (CCMP3105-4^a); Alexandrium ostenfeldii (OF101-1); Alexandrium pacificum (CCMP1598-1); Alexandrium tamarense (CCMP1771-4^a); Alexandrium tamarense (ATSP1B-1); Amphidinium carterae (CCMP1314-3^a); Azadinium spinosum (3D9-3^a); Brandtodinium nutriculum (RCC3387-1); Ceratium fusus (PA161109-2^a); Durinskia baltica (CS/38-2^a); Gambierdiscus australes (CAWD149-1); Gambierdiscus belizeanus (CCMP401-1); Glenodinium foliaceum (CCAP1116-2^a); Gonyaulax spinifera (CCMP409-1); Gymnodinium catenatum (GC744-1); Heterocapsa arctica (CCMP445-1); Heterocapsa rotundata (SCCAPK/0483-1); Heterocapsa triquestra (CCMP448-1); Karenia brevis (CCMP2229-4^a; Wilson-4^a; SP3-2^a; SP1-2^a); Karlodinium micrum (CCMP2283-3^a); Kruptoperidinium foliaceum (CCMP1326-2^a); Lingulodinium polyerda (CCMP1738-4^a); Pelagodinium beii (RCC1491-1); Peridinium aciculiferum (PEAR2-2^a); Polarella glacialis (CCMP1383-1); Prorocentrum minimum (CCMP2233-3^a); Protoceratium reticulatum (CCMP535-1); Pyrodinium bahamense (PBAHA01-1); Scrippsiella hangoei (SHTV5-3^a); Scrippsiella hangoei like (SHHI4-3^a); Scrippsiella trochoidea (CCMP3099-3^a); Symbiodinium spp (CladeA-1; Ccmp421-4^a; CassKb8-1; C1-2^a; C15-2^a; CCMP2430-3^a; D1A-1; MP-4^a; Togula jolla (CCCM725-1)

Apicomplexa: Eimeria falciformis (Bayer/Haberkorn /970-1); Eimeria tenella (Houghton-1); Neospora caninum (Liverpool-1); Toxoplasma gondii (GT1-1)

Others: Vitrella brassicaformis-CCMP3346-1); Chromera velia (CCMP2878-1)

Dinoflagellates (92, 46, 24);

Apicomplexa (4, 4, 3); Others (2, 2, 2)

 Stramenopiles

Bacillariophytes: Amphiprora sp. (CCMP467-4^a); Amphora coffeaeformis (CCMP127-3^a); Asterionellopsis glacialis (CCMP134-4^a); Attheya septentrionalis (CCMP2084-1); Aulacoseira subarctica (CCAP1002/5-1); Chaetoceros affinis (CCMP159-4^a); Chaetoceros curvisetus (NK^-4^a); Chaetoceros debilis (MM31A/1-2^a); Chaetoceros neogracile (CCMP1317-4^a); Corethron pennatum (L29A3-2^a); Coscinodiscus wailesii (CCMP2513-1); Cyclophora tenuis (ECT3854-1); Cyclotella meneghiniana (CCMP338-1); Cylindrotheca closterium (KMMCC/B181-1); Dactyliosolen fragilissimus (NK^-1); Detonula confervacea (CCMP353-1); Ditylum brightwellii (GSO103-2^a;GSO105-2^a; GSO104-3^a); Entomoneis sp (CCMP2396-1); Eucampia antarctica (CCMP1452-1); Extubocellulus spinifer (CCMP396-4^a); Fragilariopsis kerguelensis (L26/C3-4^a; L26/C5-4^a); Grammatophora oceanica (CCMP410-1); Helicotheca tamensis (CCMP826-1); Leptocylindrus danicus (B650-1); Licmophora paradoxa (CCMP2313-1); Minutocellus polymorphus (RCC2270-1); Nitzschia punctata (CCMP561-4^a); Odontella aurita (1302/5-1); Proboscia alata (P1/D3-1); Pseudo-nitzschia australis (10249/10/AB-4^a); Rhizosolenia setigera (CCMP1694-1); Skeletonema dohrnii (SkelB-2^a); Skeletonema marinoi (skelA-2^a); Skeletonema menzelii (CCMP793-2^a); Stephanopyxis turris (CCMP815-1); Striatella unipunctata (CCMP2910-1); Synedropsis recta (CCMP1620-1); Thalassionema nitzschioides (L26/B-2^a); Thalassiosira antarctica (CCMP982-4^a); Thalassiosira gravida (GMp14c1-3^a); Thalassiosira miniscula (CCMP1093-4^a); Thalassiosira oceanica (CCMP1005-4^a); Thalassiosira rotula (GSO102-4^a); Thalassiosira rotula (CCMP3096-2^a); Thalassiosira weissflogii (CCMP1336-4^a); Thalassiothrix antarctica (L6/D1-2^a); Triceratium dubium (CCMP147-1)

Bolidophytes: Bolidomonas pacifica (CCMP1866-1); Bolidomonas sp. (RCC2347-1)

Chrysophytes: Chromulina nebulosa (UTEXLB2642-1)

Pelagophytes: Aureococcus anophagefferens (CCMP1850-4^a); Aureoumbra lagunensis (CCMP1510-4^a); Pelagomonas calceolata (CCMP1756-4^a)

Raphidophytes: Chattonella subsalsa (CCMP2191-4^a); Heterosigma akashiwo (CCMP452-4^a; CCMP2393-4^a; CCMP3107-3^a; NB-3^a)

Synurophytes: Dinobryon sp (UTEXLB2267-3^a); Ochromonas sp (CCMP1393-2^a)

Dictyochophytes: Pseudopedinella elastica (CCMP716-2^a); Chrysocystis fragilis (CCMP3189-1); Dictyocha speculum (CCMP1381-1); Florenciella parvula (CCMP2471-1); Florenciella sp (RCC1587-1); Pinguiococcus pyrenoidosus (CCMP2078-1); Rhizochromulina marina (CCMP1243-1)

Pinguiophytes: Phaeomonas parva (CCMP2877-1)

Xanthophytes: Vaucheria litorea (CCMP2940-2^a)

Bacillariophytes (111, 50, 36); Bolidophytes (2, 2, 1); Chrysophytes (1, 1, 1); Pelagophytes (12, 3, 3); Raphidophytes (18, 5, 2); Synurophytes (5, 2, 2); Dictyochophytes (8, 7, 6); Pinguiophytes (1, 1, 1); Xanthophytes (2, 1, 1)

 Rhizaria

Chlorarachniophyta: Bigelowiella longifila (CCMP242-1); Bigelowiella natans (CCMP623-1)

Haplosporidia: Chlorarachnion reptans (CCCM449-1); Gymnochlora sp (CCMP2014-1); Lotharella amoebiformis (CCMP2058-1); Lotharella oceanica (CCMP622-1); Lotharella globosa (CCCM811-2^a); Partenskyella glossopodia (RCC365-1)

Chlorarachniophyta (2, 2, 1); Haplosporidia (7, 6, 4)

 Cryptophytes

Cryptomonas curvata (CCAP979:52-1); Geminigera cryophila (CCMP2564-1);  Guillardia theta (CCMP2712-1); Hanusia phi (CCMP325-1); Hemiselmis andersenii (CCMP644-1); Hemiselmis rufescens (PCC563-1); Proteomonas sulcata (CCMP704-1); Rhodomonas sp (CCMP768-2^a)

Cryptophytes (9, 8, 7)

 Haptophytes

Calcidiscus leptoporus (RCC1130-1); Chrysochromulina brevifilum (UTEXLB985-1); Chrysochromulina polylepis (CCMP1757-4^a); Chrysochromulina rotalis (UIO044-1); Chrysoculter rhomboideus (RCC1486-1); Coccolithus pelagicus (PLY182g-1); Emiliania huxleyi (PLYM219-4^a; CCMP370-4^a); Exanthemachrysis gayraliae (RCC1523-1); Gephyrocapsa oceanica (RCC1303-4^a); Imantonia sp. (RCC918-1); Isochrysis sp. (CCMP1244-2^a); Isochrysis galbana (CCMP1323-3^a); Pavlova sp. (CCMP459-3^a); Pavlova gyrans (CCMP608-1); Pavlova lutheri (RCC1537-1); Phaeocystis antarctica (CCMP1374-1); Phaeocystis cordata (RCC1383-1); Pleurochrysis carterae (CCMP645-3^a); Prymnesium parvum (Texoma1-4^a); Scyphosphaera apsteinii (RCC1455-1)

Haptophytes (43, 21, 14)

 Viridiplantae

Chlorophyceans: Chlamydomonas euryale (CCMP219-1); Dunaliella tertiolecta (CCMP1320-3^a); Polytomella parva (SAG63/3-1)

Prasinophytes: Micromonas sp. (NEPCC29-1); Micromonas sp. (CCMP2099-2^a); Pyramimonas parkeae (CCMP726-2^a); Tetraselmis striata (LANL1001-4^a)

Trebouxiophytes: Picocystis salinarum (CCMP1897-2^a)

Chlorophyceans (5, 3, 3) Prasinophytes (9, 4, 3) Trebouxiophytes (2, 1, 1)

 Excavates

Euglenids: Eutreptiella gymnastica (CCMP1594-3^a)

Euglenids (3, 1, 1)

Negative libraries

 Alveolates

Ciliates: Anophryoides haemophila (AH6-1); Aristerostoma sp (atcc50986-1); Blepharisma japonicum (StockR1072-1); Climacostomum virens (StockW/24-1); Condylostoma magnum (COL2-1); Fabrea salina (NK^-1); Favella ehrenbergii (fehre1-1); Favella taraikaensis (FeNarragansettB-2^a); Litonotus pictus (P1-1); Platyophrya macrostoma (wh-1); Protocruzia adherens (Boccale-1); Pseudokeronopsis sp (Brazil-1; OXSARD2-1); Strombidium inclinatum (s3-1); Uronema sp (bbcil-1); Strombidinopsis sp (SopsisLIS2011-1)

Dinoflagellates: Oxyrrhis marina (LB1974-3^a), Oxyrrhis marina (NK^-4^a)

Ciliates (17, 16, 14); Dinoflagellates (7, 2, 1)

 Stramenopiles

Bicosoecids: Bicosoecid sp (ms1-1); Cafeteria roenbergensis (E410-1); Cafeteria sp (Caron-1)

Labyrinthulids & Thraustochytrids: Aplanochytrium sp (PBS07-4^a); Aplanochytrium stocchinoi (GSBS06-4^a); Aurantiochytrium limacinum (ATCCMYA1381-4^a); Schizochytrium aggregatum (ATCC28209-4^a); Thraustochytrium sp (LLF1b-2^a)

Chrysophytes: Paraphysomonas vestita (GFlagA-1); Spumella elongata (CCAP955-1); Paraphysomonas bandaiensis (Caron-1)

Bicosoecids (3, 3, 2); Labyrinthulids & Thraustochytrids (18, 5, 3); Chrysophytes (3, 3, 2)

 Rhizaria

Foraminifera: Ammonia sp (NK^-1); Elphidium margaritaceum (NK^-1); Rosalina sp (NK^-1); Sorites sp (NK^-1)

Foraminifera (4, 4, 4)

 Amoebozoa

Tubulinids: Filamoeba nolandi (NCAS231-1)

Dactylopodids: Mayorella sp (BSH0219019-1); Neoparamoeba aestuarina (SoJaBio-1); Paramoeba atlantica (CCAP15609-1); Vexillifera sp (DIVA3/564-1)

Tubulinids: (1, 1, 1); Dactylopodids (4, 4, 4)

 Excavates

Kinetoplastids: Neobodo designis (CCAP1951-1)

Kinetoplastids (1, 1, 1)

 Opisthokonts

Choanoflagellates: Acanthoeca like sp. (10tr-2^a)

Choanoflagellates (2, 1, 1)

 Others

Glaucophytes: Cyanoptyche gloeocystis (SAG497-1); Gloeochaete witrockiana (SAG4684-2^a)

Palpitomonas: Palpitomonas bilix (NIES2562-1)

Glaucophytes (3, 2, 2); Palpitomonas (1, 1, 1)

Total

(402, 213, 152)