Figure 1
Genus-level taxonomic profiling of the pyrosequencing data sets of 16S rRNA genes of heavy (fraction 7; F7) and light fractions (fraction 12, F12) from [12C2]-DMS or [13C2]-DMS incubated microcosms using (a) soil sample or (b) lake sediment sample. (c, d) Ordination of canonical variates (CV1 and CV2) produced from canonical variate analysis of pyrosequencing data at family level. Sample labels include the fraction number (F7 (HF) or F12 (LF)) and type of carbon used (12C or 13C). Numbers under each plot refer to the SIP experiment time point and replicate number. i.e. 2.1: second time point, replicate 1. Numbers in (c) represent nine different SIP fractions from the soil microcosm samples. (1) Time point 1, 12C ‘heavy’ fraction (F7), (2) Time point 1, 13C ‘heavy’ fraction (F7), (3) Time point 1, 13C ‘light’ fraction (F12), (4) Time point 2, 12C ‘heavy’ fraction (F7), (5) Time- point 2, 13C ‘heavy’ fraction (F7), (6) Time point 2, 13C ‘light’ fraction (F12), (7) Time point 3, 12C ‘heavy’ fraction (F7), (8) Time point 3, 13C ‘heavy’ fraction (F7), (9) Time point 3, 13C ‘light’ fraction (F12). Numbers in (d) represent three different treatments in the lake sediment microcosm samples. (1) 12C ‘heavy’ fraction (F7), (2) 13C ‘heavy’ fraction (F7), (3) 13C ‘light’ fraction (F12). Big dots represent the means and small dots represent the individual observations. Note that only the most predominant taxa in each fraction are colour-annotated, including Methylotenera (yellow), unclassified Methylophilaceae (green), Thiobacillus (brown), other Methylophilaceae (amber) and other Betaproteobacteria (dark green). The relative abundances of the 15 most abundant genera found in the ‘heavy’ DNA fractions identified in the pyrosequencing data are shown in the Supplementary Table 2.
