Table 1 Experimental design and numbers of replicates per DNA sample across experimental setups
Compartment | Host plant | Soil type and environment | Time course | Diversification | 454 vs Illumina | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CS_C | FS_C | FS_N | 6 weeks | 12 weeks | 28 weeks | |||||||||
rep1 | rep2 | rep1 | rep2 | rep1 | rep2 | rep1 | rep2 a | rep3 | rep2 a | |||||
Soil | — | 4 | 4 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
Rhizosphere | A. alpina (Paj) | 4 | 5 | — | 3 | 3 | 4 | 4 | 4 | 4 | — | — | — | — |
A. alpina (pep1) | — | — | — | 3 | 3 | 4 | 4 | 4 | 4 | — | — | — | — | |
A. alpina (Gal5) | — | 5 | — | — | — | — | — | — | — | — | — | — | — | |
A. alpina (Gal60) | — | 5 | 5 | — | — | — | — | — | — | — | — | — | — | |
Root | A. thaliana (Col-0) | — | — | — | — | — | — | — | — | — | 3 | 3 | 3 | 3 |
C. hirsuta (Ox) | — | — | — | — | — | — | — | — | — | 3 | 3 | 3 | 2 | |
A. alpina (Paj) | 4 | 5 | — | 3 | 3 | 4 | 4 | 4 | 4 | 3 | 3 | 3 | 3 | |
A. alpina (pep1) | — | — | — | 3 | 3 | 4 | 4 | 4 | 4 | — | — | — | — | |
A. alpina (Gal5) | — | 5 | — | — | — | — | — | — | — | — | — | — | — | |
A. alpina (Gal60) | — | 5 | 5 | — | — | — | — | — | — | — | — | — | — | |
Soil type | Cologne | France | Cologne | Cologne | Cologne | |||||||||
Residence time | 12 weeks | 12 weeks | ND | 6 weeks | 6 weeks | 12 weeks | 12 weeks | 28 weeks | 28 weeks | 6 weeks | 6 weeks | 6 weeks | 6 weeks | |
Harvest date soil | Spring13 | Fall12 | Spring13 | Fall13 | Spring13 | Fall13 | Spring13 | Fall13 | Fall10 | Spring13 | Fall13 | Spring13 | ||
Environment | Controlled | Native | Controlled | Controlled | Controlled | |||||||||
Seq. technology | Illumina | Illumina | 454 | Illumina | ||||||||||
