Figure 3

Different BS11 populations in rumen fluid are associated with different size fractions. Maximum likelihood tree of the reconstructed near full-length BS11 16S rRNA gene OTUs (circles, 97% similarity). Branches are named by corresponding Silva accession number, with Prevotella (EU259391) as the outgroup. Network analyses (right) highlight the distribution of the reconstructed 16S rRNA sequences in the different size fractions (diamonds). Circle size represents the 16S rRNA gene average relative abundance across the detected fractions (detailed in Supplementary Table S2). The gray box indicates sequences corresponding to Candidatus Alcium, whereas the white box indicates sequences corresponding to Candidatus Hemicellulyticus. The blue and red stars indicate the EMIRGE 16S sequence that match identically over the V4 region to the enriched BS11 OTUs in Figure 2b (blue and red stars, respectively). In addition, the red starred sequence was also recovered from the Candidatus Alcium genome scaffolds (Supplementary Text; Supplementary Figure S11).