Figure 1
From: Bacteria isolated from lung modulate asthma susceptibility in mice
HDM-induced asthma is not exacerbated in GF mice. (a) Schematic outline of the experimental approach. Animals were killed on d16. (b) Lungs were fixed, embedded in paraffin and sectioned at a thickness of 5 μm. Lung sections were stained with hematoxylin–eosin–saffron (HES) and photographed using Case Viewer software. One representative section per group is shown (n=6 GF mice, n=11 SPF mice). The scale bar represents 50 μm. (c) BAL cells were enumerated, cytocentrifuged and stained with May–Gründwald–Giemsa. Eosinophils were enumerated and expressed as the percentage of total BAL cells. BAL IL-5 and serum IgE levels were measured by enzyme-linked immunosorbent assay (ELISA). Data are shown individually and as the mean±s.e.m. (d) Individual respiratory lymph node (RLN) cells were isolated and cultivated 72 h in RPMI with or without HDM. IL-5 and IL-10 levels in the supernatants were measured by ELISA. The RPMI±HDM values for the same mice are connected with a dotted line. Data are shown individually and as the mean±s.e.m. (a–d) All data represent one of two independent experiments (n=4–8 mice per group).
