Table 1 Genome statistics for the binned prokaryotic and eukaryotic populations and quality control statistics

Bin	Sequence size	No. contigs	% GC	Shortest contig	Mean sequence size	Longest contig	N50
Genome statistics for the binned prokaryotic and eukaryotic populations
Prolixibacteraceae	4 292 540	45	32.4	537	95 389.8	1448797	505417
Marinifilum	7 836 933	1089	37.8	601	7196.4	1053175	150714
Fusibacter	14 684 666	411	37.3	511	35 729.1	641269	190630
Fucophilus	4 491 245	124	54.3	627	36 219.7	3997856	3993360
Desulfovibrio	3 776 536	24	49.9	507	157 355.7	1377773	1119696
Desulfotalea	4 344 744	141	39.7	501	30 813.8	773452	260248
Desulfofaba	8 603 717	374	56.1	516	23 004.6	357581	54841
Carpediemonas	12 382 280	2326	58.6	500	5323.4	58509	9593
Bacillales	19 354 553	3057	32.3	500	6331.2	1002323	30666
Archaea	1 747 950	555	27	522	3149.5	61962	12541

Bin	Marker lineage	No. of genomes	No. of markers	No. of marker sets	% Completeness	Contaminations	Strain heterogeneity
Quality control statistics
Prolixibacteraceae	Bacteroidetes	419	286	195	97.95	3.08	0
Marinifilum	Bacteroidetes	419	286	195	95.9	66.9	0.52
Fusibacter	Clostridia	446	196	110	100	206.57	4.98
Fucophilus	Verrucomicrobia	12	346	245	99.59	6.71	0
Desulfovibrio	Deltaproteobacteria	93	197	125	96.66	0	0
Desulfotalea	Deltaproteobacteria	93	197	125	99.6	6.78	7.32
Desulfofaba	Deltaproteobacteria	93	197	125	96	2.6	0
Bacilliales	Bacilli	821	250	136	96.69	449.99	3.3
Archaea	Archaea	207	149	107	72.9	4.67	14.29

The marker lineages indicate the taxonomic rank of the lineage-specific marker set used to estimate quality control statistics. Genome completeness was estimated from the presence/absence of lineage specific marker genes. Contaminations were determined by the presence of multi-copy marker genes and the expected collocalization of these genes. Strain heterogeneity was determined by the number of multi-copy marker, which exceed a specified amino acid identity threshold. (See also Parks et al. (2015) for detailed explanation).

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