Figure 6
Bartonella virulence factors in B. apis and B. tamiae. (a) Distribution of 88 virulence factors in B. apis and B. tamiae categorized according to their evolutionary history (conserved in Rhizobiales versus Bartonella-specific). (b) The YadA-like trimeric autotransporter gene badA of B. henselae Houston-1 (GenBank accession CAF26961) compared with three homologous genes or gene fragments identified on different contigs in the draft genome of B. tamiae Th239. Protein domains are shown in different colors according to the legend and based on Riess et al., (2004). Contig names are indicated below the gene representations. AIMB01000003 and AIMB01000004 are adjacent contigs suggesting that the two open reading frames may present the N- and C-terminal part of the gene. In strain Th239, but not in strain Th307, the gene on contig AIMB01000003 has a frameshift in a homopolymeric G stretch as indicated by an asterisk. Vertical lines show contig ends. Diagonal dashes indicate that the contig would continue. TBLASTX hits are shown between the homologs of B. henselae and B. tamiae, with gray intensity reflecting the percentage identity of a hit (e-value cutoff=10−3). (c) Maximum likelihood phylogeny (model: WAG) of the hemin-binding proteins (Hbps) of Bartonella and corresponding homologs identified in outgroup species. The tree is based on the protein alignment (273 aa) of the conserved C-terminal region. The alignment was stripped of alignment positions with less than 50% coverage. Colors indicate different Bartonella species. A tree including the Hbps identified in all analyzed Bartonella species is shown in Supplementary Figure 13a. Bar indicates number of substitutions per site.
