Table 1 Summary of the validation of Euo target sites from ChIP-Seq by EMSA

From: Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum

Gene

Predicted function

Synonym

Shift

wcw_0093

Flagellar biosynthesis pathway, component FliP

sctR

++++

wcw_0203

Histone H1-like protein Hc1

hctA

wcw_0354

N-acetylmuramoyl-l-alanine amidase

amiA

+++

wcw_0365

Enzyme related to GTP cyclohydrolase I

queF

++++

wcw_0390

Rhs family protein

rhs9

++++

wcw_0423

Signal recognition particle GTPase

ftsY

+++

wcw_0430

NADH dehydrogenase, FAD-containing subunit

ndh

++++

wcw_0544

Integrase

wcw_0544

wcw_0547

Transposase

wcw_0547

+

wcw_0592

DNA-directed RNA polymerase, beta subunit/140 kD subunit

rpoB

+++

wcw_0655

Catalase

katA

++++

wcw_0685

ATP-dependent Zn proteases

ftsH

+

wcw_1215

Outer membrane protein

 

+

wcw_1315

Hypothetical protein

OmpA9

++

wcw_1386

UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase

murG

+

wcw_1561

Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

parC

++

wcw_1636

Transcriptional regulator of heat shock gene

hrcA

++++

wcw_1705

Predicted ATPase or kinase

wcw_1705

+++

wcw_1731

Na+/H+-dicarboxylate symporters

gltT

++++

wcw_1924

Secreted protein

 

++++

wcw_1931

Cell division protein FtsI/penicillin-binding protein 2

ftsI

+++

  1. Abbreviations: ChIP-Seq, chromatin-immunoprecipitation-sequencing; EMSA, electrophoretic mobility shift assay. ++++ for completely shifted at 1800 nm; +++ for shifted band bigger than the PCR band at 1800 nm; ++ for shifted band appears at 240 nm; +for shifted band appears at 960 nm; − for no shifted band observed.
  2. List of target promoters used as probes to test for binding by His6-Euo by EMSA. The number of plus symbols indicates the efficiency of binding.