Figure 2
From: Local diversity of heathland Cercozoa explored by in-depth sequencing
(a) Number of OTUs in 193 named cercozoan morpho-species as a function of OTU separation threshold of the V4 region. (b) Histogram of the pairwise comparisons of the V4 region from 193 named cercozoan morpho-species. The dashed red line shows our separation threshold of 5%. The bulk of the interspecific diversity is well above dissimilarities of 3 or 5%, but it extends all the way down to zero with no separation window. (c) Number of OTUs as a function of OTU separation threshold in the cleaned 454 data set of 443 350 V4 sequences. Choosing a separation threshold of 0.5–1% consistent with the morpho-species concepts would retrieve >9000 OTUs. (d) Rarefaction curve of the observed species (OTUs at 5% threshold) with error bars from combining the two drought and control plots, resampled to 85 305 sequences per tag. The samples are reasonably saturated with no difference in raw diversity between the two treatments.
