Figure 5 | The ISME Journal

Figure 5

From: Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters

Figure 5

Phylogenetic analysis of Archaea amoA sequences retrieved from the study area. Partial sequences (359 bp) of Thaumarchaeota amoA genes obtained by high-throughput sequencing were aligned against the Pester et al. (2012) database and the neighbor-joining tree was constructed in ARB. OTUs were defined at 97% similarity. Numbers following each OTU give the number of sequences and % of total sequences it represents. Additional notations apply to four major clades to indicate the % of sequences in these clades from each water mass sampled (AASW—green; WW—blue; CDW—red). Shading of trapezoids representing sequences assigned to four major clades indicates the relative contribution of sequences from AASW+WW (blue) versus all CDW (red) to these clades. Dashed boxes delineate archaeal amoA groups A (surface water) and B (deep water) as defined in Francis et al. (2005). Only bootstrap values 50% (of 1000 iterations) are shown.

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