Figure 1
From: Cold adaptation regulated by cryptic prophage excision in Shewanella oneidensis
Prophage CP4So inserted in ssrA. (a) The structure of 3′-end of the wild-type SsrA and the mutated SsrA (SsrA-delU) is predicted by ARAGORN. Mutated SsrA lacks the wobble base pair (G·U) at the tRNA-like domain of the 3′-end. (b) Prophage CP4So inserted in ssrA was identified by the presence of P4-like integrase gene and two homologous attachment sites. Site-specific recombination occurs through the cross-over between attL and attR sites and generate ΔCP4So and phage-like circle CP4Socircle, which is lost subsequently. Genes neighboring prophage CP4So are shown in blue. The attL attachment site within the ssrA gene is indicated as blue bar and the attR attachment indicated as brown bar. DNA sequences of the attP, attB, attL and attR are shown below. The deletion of a ‘T’ at position 349 in ssrA is predicted due to site-specific recombination of attL and attR during prophage excision.
