Table 1 Bacterial strains and plasmids

S. oneidensis strains

 Wild-type

MR-1

ATCC 700550

 ΔCP4So

The whole CP4So excised in MR-1

This study

 ΔssrA

Full deletion of ssrA gene in MR-1

This study

 Δhns

Full deletion of hns gene in MR-1

This study

 ΔalpA

Full deletion of alpA gene in MR-1

This study

 ΔintA

Full deletion of intA gene in MR-1

This study

E. coli strains

 K-12 BW25113

lacI^q rrnB_T14 ΔlacZ_WJ16 hsdR514 Δara BAD_AH33 Δrha BAD_LD78

(Baba et al., 2006)

 WM3064

thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360Δ(araBAD)567 ΔdapA1341::[erm pir(wt)]

(Shi et al., 2013)

 BL21(DE3)

F⁻ompT hsdS_B(r_B⁻m_B⁻) gal dcm λ(DE3) Ω P_tacUV5::T7 polymerase

Novagen

Plasmids

 pHGE

pHGE-P_tac, Km^R, IPTG-inducible expression plasmid

(Shi et al., 2013)

 pHGE-ssrA

Km^R; plasmid for expressing the wild-type ssrA

This study

 pHGE-ssrA-delU

Km^R; plasmid for expressing the mutated ssrA

This study

 pHGE-alpA

Km^R; expression plasmid for alpA of MR-1

This study

 pHGE-intA

Km^R; expression plasmid for intA of MR-1

This study

 pHGM01

Gm^R; Cm^R; Ap^R; sacB; Ori-R6K; suicide plasmid for generating in-frame deletions

(Jin et al., 2013)

 pET28b-hns

Km^R, lacI^q, pET28b P_T7-lac:: hns with C-terminal His-tagged

This study