Figure 2

Chemoautotrophic growth of “Ca. T. autotrophicus” strain EF1 under aerobic and anaerobic growth conditions. (a) Aerobic growth on copiotrophic seawater media from Puget Sound amended with 1mM S2O32−. (b) Anaerobic growth on seawater media bubbled with either N2 only or N2:CO2 gas mix and amended with 1 mM S2O32− and 100 μm NO3−. The controls were not amended with S2O32−, NO3− or CO2. Experimental treatments were conducted in triplicate.