Table 1 Overview of number of samples, number of 16S rRNA gene sequences retrieved and corresponding diversity indices (average±s.d.) for each sampling group within two experiments (natural dynamics and disturbance experiment)

From: The microbiome of coral surface mucus has a key role in mediating holobiont health and survival upon disturbance

Sampling group

Total no. of samples

No. of sequences a

Richness

Evenness

Shannon index

OTUs in total a

Chao estimate a

Natural dynamics

 New mucus

14

4882±2534

50±20

0.49±0.19

1.95±0.38

174

256

 Aged mucus

6

5867±2770

82±29

0.81±0.05

3.48±0.90

178

248

 Seawater

6

7709±3758

28±8

0.59±0.15

2.07±0.52

66

88

 Sediment

4

923±116

70±12

0.80±0.05

3.40±0.30

137

210

Disturbance experiment

 Initial composition

12

5941±2962

54±12

0.55±0.14

2.23±0.72

170

244

 Aquaria treated

6

3837±2436

50±12

0.75±0.07

2.33±0.45

109

155

 Aquaria control

6

4122±3249

84±19

0.81±0.05

3.32±0.47

176

230

 1–3 Days treatedb

18

7000±3728

67±17

0.68±0.10

2.84±0.54

201

316

 1–3 Days controlb

18

5494±1563

81±18

0.71±0.11

3.42±0.34

231

309

 4–28 Days treatedb

10

5528±3499

46±20

0.70±0.13

2.64±0.67

138

186

 4–28 Days controlb

10

8003±2583

71±15

0.78±0.06

3.17±0.43

183

315

  1. All data are based on a rarefied OTU table at the family level from which chloroplast-affiliated OTUs were removed.
  2. Abbreviations: OTU, operational taxonomic unit; rRNA, ribosomal RNA.
  3. a‘No. of sequences’, ‘OTUs in total’ and ‘Chao estimate’ were calculated from the original non-rarefied OTU data set (including chloroplasts).
  4. b1–3 and 4–28 days represent the number of days after re-introduction on the reef. Data were pooled for each of those two periods.
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