Figure 4
From: A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana
Cell size in T. pseudonana under experimental evolution conditions. (a) cell diameter measured as average particle diameter by coulter counter of cells grown for 300 generations at 22 °C (green) and 9 °C (blue). Error bars represent s.d. of measurements taken from biological triplicate samples. (b) Transcript abundance of silacidins; Protein IDs 7349, 23671, 6681, 12137 and 23686 found to be differentially requlated in TSD lines under late exponential and silicon-starved conditions and with expression patterns apparently similar to silacidins; cell wall genes silaffin 1 and silaffin 3; and an actin-like gene used as a housekeeping gene in transcriptomes of the same cell lines harvested after 300 generations at 22 °C (T300 22 °C, green), 9 °C (T300 9 °C, blue) and from batch culture nutrient conditions prior to the onset of the evolution experiment (T0 22 °C, green, with black outline). Error bars represent s.d. between transcriptomes sequenced from biological replicate samples (T300 22 °C n=5, T30 9 °C n=4).
