Figure 5
From: Sulfide production and oxidation by heterotrophic bacteria under aerobic conditions
Sulfide oxidation by mixed bacterial cells with SQR or PDO. (a) Combination of C. vitaeruminis DSM20294 (Cv) (with sqr) and S. meliloti 1021 (Sm) (with pdo). (b) Combination of E. coli BL21(DE3) (Ec) with Cpsqr [Ec(Cpsqr)] or Cppdo2 [(Ec(Cppdo2)]. Cv and Sm were induced with sulfide before harvesting, and the recombinant E. coli cells were induced with IPTG before harvesting. Cells were suspended in 50 mm Tris buffer, pH 8.0, with 50 μm DTPA at OD600nm of 2 for individual strains or 4 for mixed strains and 1 mm NaHS was added to initiate the reaction. Controls were done with individual bacterial strains. Sulfide oxidation and the production of polysulfide, sulfite and thiosulfate were detected. All data are average of three samples with standard deviation (error bar).
