Figure 2

Total copies of Syntrophomonas 16S rRNA genes measured by qPCR for each density-gradient fraction recovered from isopycnic separation of DNA from 13C-incubated microcosms and 12C-controls for both anaerobic codigesters. The filled circles indicate gradient fractions that were pooled for subsequent 16S rRNA gene amplicon sequencing and metagenomic sequencing. Both biological replicate microcosms are shown, and each point represents an average of duplicate technical replicates.