Figure 2
From: Chance and necessity in the genome evolution of endosymbiotic bacteria of insects

Experimental evolution of Escherichia coli under two population dynamics. We evolved two independent clonal lines (A and B) and five population lines (P1–P5) derived from a single ancestral population of a hypermutagenic strain of E. coli lacking the repair gene mutS under strong population bottlenecks and rich growth medium. Evolution proceeded with daily passaging a single colony to a new plate for 250 days in line A and 260 days in line B or by daily passaging 1% (100 μl) of populations P1–P5 to fresh LB broth (10 ml). Genomes were sequenced at the end of the evolution experiment.